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Title: Hepatic immunohistochemical staining with a monoclonal antibody against HCV-E2 to evaluate antiviral therapy and reinfection of liver grafts in hepatitis C viral infection
Authors: Verslype, Chris ×
Nevens, Frederik
Sinelli, Nicoletta
Clarysse, Carine
Pirenne, Jacques
Depla, Eric
Maertens, Geert
van Pelt, Jos
Desmet, Valeer
Fevery, Johan
Roskams, Tania #
Issue Date: Jan-2003
Series Title: Journal of hepatology vol:38 issue:2 pages:208-14
Abstract: BACKGROUND/AIMS: A simple and reproducible hepatic immunohistochemical staining (IHS) for hepatitis C virus (HCV) is not available. We aimed to validate hepatic IHS with monoclonal antibody (Mab) IG222, directed against the HCV-envelope 2 (E2) protein. METHODS: A three-step indirect immunoperoxidase method was used for frozen sections and a two-step indirect EnVision technique was used for paraffin-embedded sections. RESULTS: Naturally or in vitro HCV infected primary human hepatocytes were immunoreactive to HCV-E2. In the patient study (n=253), IHS had a sensitivity of 96% and a specificity of 91%. Six patients who showed positivity in the liver with Mab IG222, but remained anti-HCV and HCV-RNA negative, had hepatitis C-like changes in their liver biopsy. In one patient HCV-RNA could be detected in the liver biopsy. We confirmed early graft reinfection in patients transplanted for HCV-related disease (34 patients with serial biopsies). Treatment for acute cellular rejection with steroids was associated with an increase in staining intensity. In nine patients with clearance of HCV-RNA during antiviral therapy, seven achieved negativation of immunoreactivity and two a marked reduction. CONCLUSIONS: The IHS with Mab IG222 is an accurate tool for diagnosis and clinical management of chronic hepatitis C.
ISSN: 0168-8278
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Hepatology
Hematology Section (-)
Laboratory of Abdominal Transplantation
Translational Cell & Tissue Research
Laboratory of Nephrology
× corresponding author
# (joint) last author

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