Title: Modelling the individual cell lag phase. Isolating single cells: protocol development
Authors: Francois, K ×
Devlieghere, F
Standaert, AR
Geeraerd, Annemie
Van Impe, Jan
Debevere, J #
Issue Date: 2003
Series Title: Letters in applied microbiology vol:37 issue:1 pages:26-30
Conference: date:Katholieke Univ Leuven, BioTeC Bioproc Technol & Control, B-3001 Louvain, Belgium
Abstract: Aims: To develop a protocol to isolate single cells in wells of a microtitre plate, having a high certainty of individual cells, combined with a sufficient yield. Methods and Results: Single cells were obtained using 1/2 dilution series in microtitre plates. Seventy-two Lactococcus lactis dilution series were checked by plate counting. When the last five columns of the plates were observed, the chance of having one single cell was 80%, while the yield was 75 wells containing cells. A simulation model confirmed these results. This method was compared with the commonly applied method. Conclusions: This method makes it possible to combine a higher chance of having one cell in a microtitre well with a slightly higher yield. Significance and Impact of the Study: A tool is developed to isolate single cells to provide a suitable base for investigating and modelling the individual cell lag phase.
ISSN: 0266-8254
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
Bio- & Chemical Systems Technology, Reactor Engineering and Safety Section
× corresponding author
# (joint) last author

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