Title: In vivo activation of chicken macrophages by infectious bursal disease virus
Authors: Palmquist, J. M ×
Khatri, M
Cha, R. M
Goddeeris, Bruno
Walcheck, B
Sharma, J. M #
Issue Date: 2006
Series Title: Viral immunology vol:19 issue:2 pages:305-315
Conference: date:Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN USA; Univ Ghent, Fac Med Vet, Lab Vet Immunol, Merelbeke, Belgium; Katholieke Univ Leuven, Fac Appl Biosci & Engn, Dept Anim Sci, B-3000 Louvain, Belgium
Abstract: Infectious bursal disease virus (IBDV) infects and replicates in the dividing B lymphocytes of chickens. In the present study, the in vivo effect of IBDV infection on chicken macrophage populations and macrophage activation were examined. Specific-pathogen-free chickens were exposed to virulent IBDV and splenic macrophages were recovered during the acute phase of the disease. At 3 and 5 days post-infection (dpi), spleens of virus-exposed chickens had fewer macrophages than those of virus-free controls (p < 0.05). Confocal microscopic examination revealed cells that were positive for both KUL01 (macrophage surface marker) and R63 (IBDV-VP2), indicating presence of the virus in macrophages. MQ-NCSU cells, an avian macrophage cell line, were susceptible to replication of IBDV. In addition, splenic macrophages were activated and had temporarily increased levels of mRNA transcripts of pro-inflammatory mediators, including IL-1`beta, IL-6, IL-18, and NOS. The robust expression of proinflammatory cytokine transcripts, along with a decrease in macrophage numbers, suggest that IBDV activates and may lead to a reduction of resident macrophages in vivo.
ISSN: 0882-8245
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
× corresponding author
# (joint) last author

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