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Title: Vascular and cellular proteolytic activity in mice with alpha(2)-antiplasmin gene inactivation
Authors: Lijnen, Roger ×
Ugwu, Francisca
Maquoi, Erik
Lemmens, Gudula
Van Hoef, B
Dewerchin, Mieke
Collen, Desire #
Issue Date: 2000
Series Title: Fibrinolysis: an international journal of fibrinolysis and thrombolysis vol:14 issue:5 pages:322-326
Conference: date:Univ Leuven, Ctr Mol & Vasc Biol, B-3000 Louvain, Belgium; Flanders Interuniv Inst Biotechnol, Ctr Transgene Technol & Gene Therapy, Louvain, Belgium
Abstract: Objective: To study the role of alpha(2)-antiplasmin (alpha(2)-AP), the main physiological plasmin inhibitor, in controlling vascular and cellular proteolytic activity. Materials: Arteries, organs and cell cultures derived from alpha(2)-AP-deficient (alpha(2)-AP(-/-)) mice or from their wild-type littermates (alpha(2)-AP(+/+)). Results: In serum-free conditioned medium of alpha(2)-AP(+/+) or alpha(2)-AP(-/-) skin fibroblasts, the time course (0-72 h) of PAI-1 antigen and of t-PA or u-PA antigen and activity production was similar. Activation of proMMP-9 (gelatinase B) upon addition of plasmin(ogen) to serum-free conditioned medium of fibroblasts was consistently detectable with alpha(2)-AP(-/-) but not with alpha(2)-AP(+/+) cells. In aorta and femoral arterial extracts of alpha(2)-AP(+/+) or alpha(2)-AP(-/-) mice, t-PA and u-PA activity levels were comparable, and fibrin zymography with cryosections did not reveal significant differences in fibrinolytic activity. In liver or kidney extracts of alpha(2)-AP(+/+) or alpha(2)-AP(-/-) mice, t-PA, u-PA, PAI-1 and plasminogen antigen levels were comparable; t-PA or u-PA activity was not detected in liver extracts and was present at comparable levels in kidney extracts. Activation of plasminogen to plasmin in solution by cell-associated plasminogen activator, and activation of cell-bound plasminogen by tcu-PA was comparable for fibroblasts of both genotypes. Conclusions: alpha(2)-AP does not play a crucial role in controlling vascular or cellular proteolytic activity in mice. (C) 2000 Harcourt Publishers Ltd.
ISSN: 0268-9499
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
Laboratory of Angiogenesis and Vascular Metabolism (VIB-KU Leuven Centre for Cancer Biology) (+)
× corresponding author
# (joint) last author

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