Title: Cytokine production following experimental implantation of xenogenic dermal collagen and polypropylene grafts in mice
Authors: Zheng, Fang ×
Xu, Luo
Verbiest, Lieve
Verbeken, Eric
De Ridder, Dirk
Deprest, Jan #
Issue Date: 2007
Publisher: Allen R. Liss
Series Title: Neurourology and urodynamics vol:26 issue:2 pages:280-9
Abstract: AIM: We earlier showed that xenogenic Pelvicol (Bard, Olen, Belgium) implants induce a lesser inflammatory response than Prolene (Johnson and Johnson, Dilbeek, Belgium). The purpose of this study was to determine cytokine profiles in the host immune responses to Pelvicol in a mouse model. The hypothesis was that Pelvicol would induce a "T-helper2" (Th2) rather than T-helper1 (Th1) type of inflammatory response. METHODS: Mice were implanted subcutaneously with Pelvicol or Prolene and the graft sites were harvested at 3 to 28 days. Histopathology was done and cytokine levels were determined by immunohistochemistry and RT-PCR. Flow cytometry was used to identify which cell population contributed to the observed cytokine production profiles. RESULTS: Pelvicol induced a decreased inflammation and displayed an increase in IL-10 and TGF-beta, but reduce of TNF-alpha and IFN-gamma, indicating a Th2 type dominated response as examined by immunohistochemistry and RT-PCR. Flow cytometry showed that the monocytes/maceophages were the main cell population responsible for production of these cytokines. Monocytes/maceophages from Pelvicol explants showed upregulated expression of IL-10 while Prolene explants expressed TNF-alpha. CONCLUSION: Pelvicol induced a Th2 type cytokine-dominated immune response after subcutaneous implantation in mice. Neurourol. Urodynam. (c) 2006 Wiley-Liss, Inc.
ISSN: 0733-2467
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Cell & Tissue Research
Basic Research in Gynaecology Section (-)
Urology Section (-)
Foetal Medicine Section (-)
× corresponding author
# (joint) last author

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