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Title: Trisomy 7 and trisomy 10 characterize subpopulations of tumor-infiltrating lymphocytes in kidney tumors and in the surrounding kidney tissue
Authors: Dal Cin, Paola ×
Aly, Magda S.
Delabie, Jan
Ceuppens, Jan
Van Gool, Stefaan
Van Damme, Boudewijn
Baert, Luc
Van Poppel, Hendrik
Van Den Berghe, Herman #
Issue Date: Oct-1992
Series Title: Proceedings of the National Academy of Sciences of the United States of America vol:89 issue:20 pages:9744-9748
Abstract: We performed conventional cytogenetic analysis and fluorescence in situ hybridization in short-term cultures of normal and neoplastic kidney tissues. Cell populations carrying an extra chromosome 7 or an extra chromosome 10 as the only chromosome change could be identified in kidney tumors, mostly renal cell carcinomas, and in the surrounding kidney tissue, but not in nonneoplastic kidneys. To identify the type of cells displaying these aneuploidies, we performed in situ hybridization (ISH) with probes specific for the centromeric region of chromosomes 7 and 10 on frozen kidney tissue sections. Trisomy 7 and trisomy 10 were restricted to infiltrating inflammatory cells in the tumor as well as in the surrounding tissue. Trisomy 7 and trisomy 10 were also found in subpopulations of peripheral blood T cells of cancer patients and of normal individuals, as well as in the thymus of five normal fetuses (21-29 weeks), but not in noninvaded reactive lymph node sections of patients without malignancy. When lymphocytes were enriched from kidney tumors and surrounding tissue by either Ficoll/Hypaque density gradient or immunomagnetic selection with anti-CD3, anti-CD4, or anti-CD8 monoclonal antibodies, it was confirmed that they contained a high percentage of trisomy 7 and trisomy 10 cells. Further proof for T-lymphocyte origin of the trisomy 7 and trisomy 10 cells was obtained by simultaneous staining of lymphocytes isolated from tumor tissue with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and ISH. We conclude that trisomy 7 and trisomy 10, found in renal carcinomas and surrounding kidney tissue, characterize subpopulations of tumor-infiltrating lymphocytes. The biologic significance of this phenomenon is unknown and requires further investigation.
ISSN: 0027-8424
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Cell & Tissue Research
Urology Section (-)
Laboratory of Clinical Immunology
Clinical Genetics Section (-)
Department of Human Genetics - miscellaneous
Laboratory of Pediatric Immunology
× corresponding author
# (joint) last author

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