Title: In vitro growth of mastitis-inducing Escherichia coli in milk and milk fractions of dairy cows
Authors: Kornalijnslijper, JE ×
van Werven, T
Daemen, AJJM
van den Broek, J
Niewold, Theodoor
Rutten, VPMG
Noordhuizen-Stassen, EN #
Issue Date: 2003
Series Title: Veterinary microbiology vol:91 issue:2-3 pages:125-134
Conference: date:Univ Utrecht, Fac Vet Med, Ruminant Hlth Unit, Dept Farm Anim Hlth, NL-3584 CL Utrecht, Netherlands; Inst Anim Sci & Hlth, Div Anim Sci, NL-8200 AB Lelystad, Netherlands; Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Div Immunol, NL-3584 CL Utrecht, Netherlands
Abstract: The outcome of E. coli mastitis in cows ranges from mild to severe in individual animals. This study explored the hypothesis that milk from individual cows differs in its growth medium properties for E. coli, and whether possible variation could be related to specific milk constituents. To mimic the early phase of intramammary E. coli infection, a low inoculum size and a short incubation period were used. Cell-reduced, cell- and fat-free (skim) and cell- and fat-free and protein-reduced (whey) fractions were prepared from whole milk samples (n = 18). Ten ml of whole milk, milk fractions and brain heart infusion broth (BRI) were inoculated with approximately 100 cfu E. coli. After 6 It of incubation, bacterial counts were assessed by dilution plating in triplicate. Bacterial counts in whole milk differed up to a 100-fold between cows, which was not associated with SCC. Bacterial counts were significantly higher in whey fractions than in whole milk, cell-reduced and skim fractions and variation in whey was smaller, indicating that the acid-precipitable protein fraction contains the milk constituents of major relevance for inhibition of and variation in bacterial growth. The presence of fat and cells added to bacterial growth inhibition to a lesser extent. In conclusion, in vitro growth of E. coli in milk differs substantially between individual cows within an incubation period comparable with the early phase of intramammary infection. This suggests that the growth medium properties of milk could be of importance in the pathogenesis of E. coli mastitis and subsequent outcome of disease. (C) 2002 Elsevier Science B.V. All rights reserved.
ISSN: 0378-1135
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

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