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Title: Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro but poorly protect against diarrhoea
Authors: Harmsen, MM ×
van Solt, CB
Hoogendoorn, A
van Zijderveld, FG
Niewold, Theodoor
van der Meulen, J #
Issue Date: 2005
Publisher: ELSEVIER SCIENCE BV
Series Title: Veterinary microbiology vol:111 issue:1-2 pages:89-98
Conference: date:Univ Wageningen & Res Ctr, Inst Anim Sci & Hlth ID Lelystad, NL-8219 PH Lelystad, Netherlands; Cent Inst Anim Dis Control, NL-8203 AA Lelystad, Netherlands
Abstract: Oral administration of polyclonal antibodies directed against enterotoxigenic Escherichia coli (ETEC) F4 fimbriae is used to protect against piglet post-weaning diarrhoea. For cost reasons, we aim to replace these polyclonal antibodies by recombinant llama single-domain antibody fragments (VHHs) that can be produced efficiently in microorganisms. Six F4 fimbriae specific VHHs were isolated. The VHH that was produced at the highest level by yeast, K609, was further analysed. 3.8 mg/L K609 inhibited 90% of bacterial attachment to intestinal brush borders in vitro. Perfusion of a jejunal segment with at least 4 mg/L K609 reduced the ETEC-induced fluid loss, but only to 30%. Preventive administration of a high K609 dose (150 mg/(piglet day)) to piglets that were challenge infected with ETEC resulted in less severe diarrhoea only at 4 and 5 days post-infection, but did not improve average daily weight gain, ETEC shedding and piglet survival. Thus, we have shown that an antibody fragment that effectively inhibited in vitro ETEC adhesion to intestinal brush borders poorly protected piglets against experimental ETEC infection. (C) 2005 Elsevier B.V. All rights reserved.
ISSN: 0378-1135
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

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