ITEM METADATA RECORD
Title: EFFECTS OF CYTOKINES, BACTERIAL-ENDOTOXIN AND HYDROCORTISONE ON PRIMARY HEPATOCYTE CULTURES OF RAT AND HAMSTER
Authors: TOUSSAINT, MJM ×
Niewold, Theodoor
DEWIT, MML #
Issue Date: 1993
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Series Title: Toxicology in Vitro vol:7 issue:4 pages:443-446
Abstract: In vivo an acute phase reaction is characterized by changes in hepatic protein metabolism and modification of liver drug enzymes (cytochrome P-450). In vitro, mediators of an acute phase reaction have been reported to have a similar effect. In the present study, the effects of bacterial endotoxin (lipopolysaccharide, LPS) and cytokines in hepatocyte cultures of rat and hamster were examined. In both species, a significant increase in secretion of specific acute phase protein was measured after 48 hr of exposure to IL-6, TNF-alpha or LPS. LPS and TNF-alpha increased the secretion of serum amyloid A (SAA) in hamster hepatocytes. LPS and IL-6 (but only in the presence of hydrocortisone) increased the secretion of alpha2-macroglobulin (alpha2M) in rat hepatocytes. An effect on the total concentration of cytochrome P-450 was not found in either species after exposure to glucocorticoids or to LPS and cytokines, although the latter compounds caused a marked increase in secretion of acute phase proteins.
ISSN: 0887-2333
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy

 




All items in Lirias are protected by copyright, with all rights reserved.

© Web of science