Title: Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination
Authors: Esguerra, Camila ×
Nelles, Lucien
Vermeire, Liesbeth
Ibrahimi, Abdelilah
Crawford, Alexander
Derua, Rita
Janssens, Els
Waelkens, Etienne
Carmeliet, Peter
Collen, Desire
Huylebroeck, Danny #
Issue Date: Dec-2007
Publisher: Company of biologists ltd
Series Title: Development vol:134 issue:24 pages:4381-4393
Abstract: During vertebrate development, signaling by the TGF beta ligand Nodal is critical for mesoderm formation, correct positioning of the anterior-posterior axis, normal anterior and midline patterning, and left-right asymmetric development of the heart and viscera. Stimulation of Alk4/EGF-CFC receptor complexes by Nodal activates Smad2/3, leading to left-sided expression of target genes that promote asymmetric placement of certain internal organs. We identified Ttrap as a novel Alk4- and Smad3-interacting protein that controls gastrulation movements and left-right axis determination in zebrafish. Morpholino-mediated Ttrap knockdown increases Smad3 activity, leading to ectopic expression of snail1a and apparent repression of e-cadherin, thereby perturbing cell movements during convergent extension, epiboly and node formation. Thus, although the role of Smad proteins in mediating Nodal signaling is well-documented, the functional characterization of Ttrap provides insight into a novel Smad partner that plays an essential role in the fine-tuning of this signal transduction cascade.
ISSN: 0950-1991
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Biology (Celgen) (-)
Molecular Virology and Gene Therapy
Laboratory for Pharmaceutical Biology (-)
Molecular and Vascular Biology
Laboratory of Protein Phosphorylation and Proteomics
Animal Physiology and Neurobiology Section - miscellaneous
Laboratory of Phosphoproteomics (-)
Laboratory of Angiogenesis and Vascular Metabolism (VIB-KU Leuven Centre for Cancer Biology) (+)
× corresponding author
# (joint) last author

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