This item still needs to be validated !
Title: An intramolecular excimer forming probe used to study the interaction of alpha-lactalbumin with model membranes
Authors: Dangreau, H ×
Joniau, Marcel
De Cuyper, Marcel
Hanssens, Ignace #
Issue Date: 20-Jun-1982
Series Title: Biochemistry vol:21 issue:15 pages:3594-8
Abstract: The nonconjugated bichromophoric molecule 1,3-di(1-pyrenyl)propane shows, besides the pyrene monomer fluorescence, a structureless emission due to an intramolecular excited dimer (excimer). In the case of intramolecular excimer forming systems, the ratio of the emission intensities of excimer vs. monomer (IE/IM) is sensitive to changes in membrane structure as will be illustrated here. The present molecule is a useful probe to report on lipid-protein interactions, at least in our model system. We have introduced it into the hydrocarbon layer of dimyristoylphosphatidylcholine vesicles in order to study their interaction with alpha-lactalbumin (alpha-LA) as a function of pH and temperature. On the basis of steady-state fluorescence, kinetic, and energy transfer studies, we have found that, at pH 4, alpha-LA strongly interacts with the lipid bilayer. In steady-state fluorescence experiments, changes of the ratio IE/IM and shifts of the transition temperature have been observed, reflecting changes of membrane structure caused by interaction with alpha-LA. Kinetic studies of the rate of interaction of alpha-lactalbumin and measurements of energy transfer from excited tryptophan(s) to the fluorescent probe confirm the steady-state experiments. Our results agree with previously reported microcalorimetric, gel chromatographic, and fluorescence polarization studies.
ISSN: 0006-2960
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Medicine, Campus Kulak Kortrijk
Faculty of Science, Campus Kulak Kortrijk
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science