Title: 3-azido-L-tyrosine as a photoinhibitor of tubulin:tyrosine ligase. Role of thiol groups
Authors: Coudijzer, K ×
Joniau, Marcel #
Issue Date: 30-Jun-1990
Series Title: FEBS Letters vol:268 issue:1 pages:95-8
Abstract: We have synthesized the photoactivatable probes 3-azido-L-tyrosine and p-azido-L-phenylalanine and studied their capacity to inhibit the incorporation of [3H]tyrosine into tubulin catalyzed by tubulin:tyrosine ligase. Without illumination, only 3-azido-L-tyrosine reversibly inhibits the enzyme. Upon illumination, both reagents irreversibly photoinactivate the enzyme in a similar way. The ligase can be protected against photoinactivation by reversibly blocking essential thiol groups with pCMB during illumination.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Medicine, Campus Kulak Kortrijk
× corresponding author
# (joint) last author

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