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Keywords:

Animals, Calcium, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Female, Horses, Isoelectric Focusing, Milk, Muramidase, Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Chemistry, Analytical, Biochemistry & Molecular Biology, Chemistry, HYDROPHOBIC-INTERACTION, CHROMATOGRAPHY, WHEY, 03 Chemical Sciences, 09 Engineering, 10 Technology, Analytical Chemistry, 34 Chemical sciences, 40 Engineering

Abstract:

A fast, simple two-step purification scheme is presented for the isolation of lysozyme at a high yield from equine milk. In the first step, fluidized bed technology, using the Streamline system, was exploited. In the following step, advantage was taken of Ca(2+)-induced conformational changes to obtain a pure, high specific activity, enzyme fraction by hydrophobic interaction chromatography.