Communications in Agricultural and Applied Biological Sciences vol:72 issue:1 pages:35-39
A growing number of Salmonella enterica serovar Typhimurium (S. Typhi-murium) strains, important agents of bacterial food-borne diseases, are be-coming multi-resistant to various antibiotics. Moreover, Salmonella is capa-ble of forming biofilms on a variety of surfaces and these Salmonella biofilms show an even increased resistance to antibiotics (Römling et al., 2007). A type of cell-cell communication mediated by signal molecules, known as quorum sensing (QS), is of importance for virulence and biofilm formation of several pathogens. S. Typhimurium is known to encode two different QS systems: it has an acyl-homoserine lactone (AHL) receptor, SdiA, to sense AHLs that are produced by other bacterial species, while it also uses LuxS to synthesize the AI-2 group of signal molecules (Ahmer, 1998; Surette et al., 1999). However, knowledge about the physiological impact of the Salmonella QS systems is currently limited. The objective of this study was to synthesize and screen limited libraries of analogues of signal molecules to identify small molecules which are activators or inhibitors of the SdiA-mediated QS system, as well as inhibitors of Salmonella biofilm formation.