Title: The combination of anti-B7 monoclonal antibody and cyclosporin A induces alloantigen-specific anergy during a primary mixed lymphocyte reaction
Authors: Van Gool, Stefaan ×
de Boer, M
Ceuppens, Jan #
Issue Date: 21-Mar-1994
Series Title: Journal of Experimental Medicine vol:179 issue:2 pages:715-20
Abstract: Interaction of CD28/CTLA-4 on T cells with B7 on antigen-presenting cells constitutes an important costimulatory signal for T cells and is responsible for cyclosporin A-resistant interleukin 2 (IL-2) gene expression and potentially also for prevention of anergy induction after T cell receptor triggering. In this paper, we demonstrate that addition of a monoclonal antibody to B7, which blocks B7-CD28/CTLA-4 interaction, and of cyclosporin A together, but not separately, to a primary mixed lymphocyte reaction of freshly isolated human T cells towards a human B cell line, induces nonresponsiveness of alloantigen-specific cytotoxic T lymphocyte precursors, whereas reactivity to a third party stimulator is intact. Nonresponsiveness could be reversed by culture in IL-2, indicating that anergy, and not clonal deletion, is responsible for this phenomenon. Our finding opens important perspectives for the development of new therapeutic strategies in transplantation.
ISSN: 0022-1007
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pediatric Hematology & Oncology Section (-)
Laboratory of Clinical Immunology
Laboratory of Pediatric Immunology
× corresponding author
# (joint) last author

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