Title: Microgel immunoblotting of thymus and nuclear extracts by unidirectional diffusion
Authors: De Keyser, F ×
Verbruggen, G
Veys, E
Luyten, Frank
Segers, J
Rabaey, M #
Issue Date: Aug-1989
Series Title: Analytical biochemistry vol:176 issue:2 pages:350-2
Abstract: A variant immunoblotting procedure is presented, starting from sodium dodecyl sulfate-containing ultra-thin microgels. The use of these gels allows efficient transfer of proteins to an immobilizing matrix such as nitrocellulose, by simple uni-directional diffusion, without loss of resolution. To this end an assembly was developed, keeping the microgel and the immobilizing matrix in continuous contact. To standardize the technique, two protein extracts (a classical rabbit thymus extract and an original autologous nuclear extract) were used, together with reference antinuclear antisera. The method is fast, easy to perform, and perfectly reproducible. For these reasons, the technique is very suitable for screening a patient's sera on a large scale.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Rheumatology Section (-)
× corresponding author
# (joint) last author

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