Title: Deletion of the transient receptor potential cation channel TRPV4 impairs murine bladder voiding
Authors: Gevaert, Thomas ×
Vriens, Joris
Segal Stanciu, Andrei
Everaerts, Wouter
Roskams, Tania
Talavera PĂ©rez, Karel
Owsianik, Grzegorz
Liedtke, Wolfgang
Daelemans, Dirk
Dewachter, Ilse
Van Leuven, Freddy
Voets, Thomas
De Ridder, Dirk
Nilius, Bernd #
Issue Date: Nov-2007
Publisher: American Society for Clinical Investigation
Series Title: Journal of Clinical Investigation vol:117 issue:11 pages:3453-3462
Abstract: Here we provide evidence for a critical role of the transient receptor potential cation channel, subfamily V, member 4 (TRPV4) in normal bladder function. Immunofluorescence demonstrated TRPV4 expression in mouse and rat urothelium and vascular endothelium, but not in other cell types of the bladder. Intracellular Ca2+ measurements on urothelial cells isolated from mice revealed a TRPV4-dependent response to the selective TRPV4 agonist 4alpha-phorbol 12,13-didecanoate and to hypotonic cell swelling. Behavioral studies demonstrated that TRPV4-/- mice manifest an incontinent phenotype but show normal exploratory activity and anxiety-related behavior. Cystometric experiments revealed that TRPV4-/- mice exhibit a lower frequency of voiding contractions as well as a higher frequency of nonvoiding contractions. Additionally, the amplitude of the spontaneous contractions in explanted bladder strips from TRPV4-/- mice was significantly reduced. Finally, a decreased intravesical stretch-evoked ATP release was found in isolated whole bladders from TRPV4-/- mice. These data demonstrate a previously unrecognized role for TRPV4 in voiding behavior, raising the possibility that TRPV4 plays a critical role in urothelium-mediated transduction of intravesical mechanical pressure.
ISSN: 0021-9738
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Urology Section (-)
Translational Cell & Tissue Research
Laboratory of Virology and Chemotherapy (Rega Institute)
Laboratory of Experimental Genetics and Transgenesis (-)
Associated Laboratories - miscellaneous (-)
Physiology Section (-)
Screening, Diagnostics and Biomarkers (-)
Department of Cellular and Molecular Medicine - miscellaneous
Laboratory of Ion Channel Research
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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