Title: Thrombophilia in mice expressing a tissue factor variant lacking its transmembrane and cytosolic domain
Authors: Melis, Els
Moons, Lieve
Arnout, Jozef
Hoylaerts, Marc
Collen, Desire
Carmeliet, Peter
Dewerchin, Mieke # ×
Issue Date: Jul-2005
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:333 issue:2 pages:488-495
Abstract: Mice with a targeted truncation in the gene encoding tissue factor of blood coagulation (TF) to eliminate the cytosolic domain and carrying a neo(R) cassette in intron 5 unexpectedly displayed severe spontaneous thrombosis in various vascular beds. Thrombosis was observed in heterozygous TF(+/neo) mice, causing death of over 50% of adults within 36 weeks of birth, and fulminantly exacerbating in pregnant females. Homozygous TF(neo/neo) mice were more severely affected and died within 7 weeks after birth. These TF(neo) mice primarily synthesized a mutant mRNA aberrantly spliced from exon 5 to neo(R), encoding an apparently non-vesicle-binding soluble TF lacking both the transmembrane and cytosolic domain, but still capable of blood coagulation induction. This severe thrombotic phenotype associated with the presence of a non-anchored soluble TF variant underscores the recently recognized significance of circulating TF for thrombus formation and development.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
Faculty of Medicine - miscellaneous
Animal Physiology and Neurobiology Section - miscellaneous
Biomedical Sciences Group Management - miscellaneous
Biomedical Sciences Teaching Methodology and Practicals
Laboratory of Angiogenesis and Vascular Metabolism (VIB-KU Leuven Centre for Cancer Biology) (+)
× corresponding author
# (joint) last author

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