Title: FVIII production by human lung microvascular endothelial cells
Authors: Jacquemin, Marc ×
Neyrinck, Arne
Hermanns, Maria Iris
Lavend'homme, Renaud
Rega, Filip
Saint-Remy, Jean-Marie
Peerlinck, Kathelijne
Van Raemdonck, Dirk
Kirkpatrick, Charles James #
Issue Date: Jul-2006
Series Title: Blood vol:108 issue:2 pages:515-517
Abstract: While extrahepatic factor VIII (FVIII) synthesis suffices for hemostasis, the extrahepatic production sites are not well defined. We therefore investigated the ability of the human lungs to produce FVIII. Lungs from heart-beating donors who were declined for transplantation were perfused and ventilated in an isolated reperfusion model for 2 hours. A progressive accumulation of FVIII and von Willebrand factor (VWF) was recorded in the perfusion medium in 3 of 4 experiments. By contrast, factor V, fibrinogen, and immunoglobulin G (IgG) levels remained constant during the perfusion period, indicating that the accumulation of FVIII and VWF was not due to diffusion from the intercellular medium into the vascular system. Purified human lung microvascular endothelial cells produced FVIII during at least 2 passages in vitro. Altogether, these data identify the lung endothelial cells as a FVIII production site in humans.
ISSN: 0006-4971
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
Facility for Surgery and Anaesthesiology
Thoracic Surgery
Cardiac Surgery
× corresponding author
# (joint) last author

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