Title: Functional expression of a locust tyramine receptor in murine erythroleukaemia cells
Authors: Poels, Jeroen ×
Suner, M M
Needham, M
Torfs, Herbert
De Rijck, J
De Loof, Arnold
Dunbar, S J
Vanden Broeck, Jozef #
Issue Date: Dec-2001
Publisher: Blackwell science ltd
Series Title: Insect molecular biology vol:10 issue:6 pages:541-548
Abstract: The LCR/MEL system (Locus Control Region/Murine Erythroleukaemia cells) was employed to express and characterize the Locusta migratoria tyramine receptor (TyrLoc), an insect G protein-coupled receptor. Functional agonist-dependent responses were recorded in stable, tyramine receptor expressing cell clones (MEL-TyrLoc). Tyramine elicited a dose-dependent increase of cytosolic Ca2+-ions and an attenuation of forskolin-induced cyclic adenosine monophosphate (AMP) production. Octopamine was shown to be a weak agonist for both responses. In addition, yohimbine proved to be a potent tyramine receptor antagonist. This study reports the first application of the LCR/MEL expression system in functional assays for G protein-coupled receptors and therefore expands the capabilities of this system by exploiting the functionality of the signal transduction pathways.
ISSN: 0962-1075
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Virology and Gene Therapy
Department of Biology - miscellaneous
Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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