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Title: A multiparametric assay for quantitative nerve regeneration evaluation
Authors: Weyn, Barbara ×
Van Remoortere, M
Nuydens, R
Meert, Theo
Van de Wouwer, G #
Issue Date: Aug-2005
Publisher: Blackwell Science
Series Title: Journal of Microscopy vol:219 pages:95-101
Abstract: We introduce an assay for the semi-automated quantification of nerve regeneration by image analysis. Digital images of histological sections of regenerated nerves are recorded using an automated inverted microscope and merged into high-resolution mosaic images representing the entire nerve. These are analysed by a dedicated image-processing package that computes nerve-specific features (e.g. nerve area, fibre count, myelinated area) and fibre-specific features (area, perimeter, myelin sheet thickness). The assay's performance and correlation of the automatically computed data with visually obtained data are determined on a set of 140 semithin sections from the distal part of a rat tibial nerve from four different experimental treatment groups (control, sham, sutured, cut) taken at seven different time points after surgery. Results show a high correlation between the manually and automatically derived data, and a high discriminative power towards treatment. Extra value is added by the large feature set. In conclusion, the assay is fast and offers data that currently can be obtained only by a combination of laborious and time-consuming tests.
URI: 
ISSN: 0022-2720
Publication status: published
KU Leuven publication type: IT
Appears in Collections:ESAT - PSI, Processing Speech and Images
Laboratory for Biological Psychology
× corresponding author
# (joint) last author

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