Title: Heterodimerization with vascular endothelial growth factor receptor-2 (VEGFR-2) is necessary for VEGFR-3 activity
Authors: Alam, Antoine ×
Herault, Jean-Pascal
Barron, Pauline
Favier, Benoit
Fons, Pierre
Delesque-Touchard, Nathalie
Senegas, Isabelle
Laboudie, Patricia
Bonnin, Jacques
Cassan, Cecile
Savi, Pierre
Ruggeri, Bruce
Carmeliet, Peter
Bono, Françoise
Herbert, Jean-Marc #
Issue Date: Oct-2004
Series Title: Biochemical and Biophysical Research Communications vol:324 issue:2 pages:909-15
Abstract: VEGFR-3 is essential for vascular development and maintenance of lymphatic vessel's integrity. Little is known about its cooperative effect with other receptors of the same family. Contrary to VEGFR-2, stimulation of VEGFR-3 by VEGF-C and -D failed to enhance its phosphorylation either in HEK293T or in PAE cells. These ligands were unable to induce angiogenesis of PAEC expressing VEGFR-3 alone. In the presence of VEGFR-2, VEGF-C and -D induced heterodimerization of VEGFR-3 with VEGFR-2. This heterodimerization was associated with enhanced VEGFR-3 phosphorylation and subsequent cellular responses as evidenced by the formation of capillary-like structures in PAE cells and proliferation of primary human endothelial cells expressing both receptors. Taken together, these results show for the first time that VEGFR-3 needs to be associated to VEGFR-2 to induce ligand-dependent cellular responses.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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