Title: A genetic Xenopus laevis tadpole model to study lymphangiogenesis
Authors: Ny, Annelii ×
Koch, Marta
Schneider, Martin
Neven, Elke
Tong, Ricky T
Maity, Sunit
Fischer, Christian
Plaisance, Stephane
Lambrechts, Diether
Héligon, Christophe
Terclavers, Sven
Ciesiolka, Malgorzata
Kälin, Roland
Man, Wing Yan
Senn, Irena
Wyns, Sabine
Lupu, Florea
Brändli, André
Vleminckx, Kris
Collen, Desire
Dewerchin, Mieke
Conway, Edward
Moons, Lieve
Jain, Rakesh K
Carmeliet, Peter #
Issue Date: Sep-2005
Publisher: Nature Pub. Co.
Series Title: Nature Medicine vol:11 issue:9 pages:998-1004
Abstract: Lymph vessels control fluid homeostasis, immunity and metastasis. Unraveling the molecular basis of lymphangiogenesis has been hampered by the lack of a small animal model that can be genetically manipulated. Here, we show that Xenopus tadpoles develop lymph vessels from lymphangioblasts or, through transdifferentiation, from venous endothelial cells. Lymphangiography showed that these lymph vessels drain lymph, through the lymph heart, to the venous circulation. Morpholino-mediated knockdown of the lymphangiogenic factor Prox1 caused lymph vessel defects and lymphedema by impairing lymphatic commitment. Knockdown of vascular endothelial growth factor C (VEGF-C) also induced lymph vessel defects and lymphedema, but primarily by affecting migration of lymphatic endothelial cells. Knockdown of VEGF-C also resulted in aberrant blood vessel formation in tadpoles. This tadpole model offers opportunities for the discovery of new regulators of lymphangiogenesis.
ISSN: 1078-8956
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Vesalius Research Centre (-)
Molecular and Vascular Biology
Physiology Section (-)
Animal Physiology and Neurobiology Section - miscellaneous
Laboratory of Neurogenetics (-)
Laboratory of Translational Genetics (Vesalius Research Center) (+)
Laboratory of Angiogenesis and Vascular Metabolism (VIB-KU Leuven Centre for Cancer Biology) (+)
× corresponding author
# (joint) last author

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