Title: Mouse Aortic Ring Assay: A New Approach of the Molecular Genetics of Angiogenesis
Authors: Masson V, V ×
Devy, Laetitia
Grignet-Debrus, Christine
Bernt, Sarah
Bajou, Khalid
Blacher, Silvia
Roland, Guy
Chang, Yawen
Fong, Timothy
Carmeliet, Peter
Foidart, Jean-Michel
Noël, Agnès #
Issue Date: 2002
Publisher: Biological Procedures Online
Series Title: Biological Procedures Online vol:4 issue:1 pages:24-31
Abstract: Angiogenesis, a key step in many physiological and pathological processes, involves proteolysis of the extracellular matrix. To study the role of two enzymatic families, serine-proteases and matrix metalloproteases in angiogenesis, we have adapted to the mouse, the aortic ring assay initially developed in the rat. The use of deficient mice allowed us to demonstrate that PAI-1 is essential for angiogenesis while the absence of an MMP, MMP-11, did not affect vessel sprouting. We report here that this model is attractive to elucidate the cellular and molecular mechanisms of angiogenesis, to identify, characterise or screen "pro- or anti-angiogenic agents that could be used for the treatment of angiogenesis-dependent diseases. Approaches include using recombinant proteins, synthetic molecules and adenovirus-mediated gene transfer.
ISSN: 1480-9222
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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