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Title: High-level expression of active HIV-1 integrase from a synthetic gene in human cells
Authors: Cherepanov, Peter
Pluymers, Wim
Claeys, Anje
Proost, Paul
De Clercq, Erik
Debyser, Zeger # ×
Issue Date: Sep-2000
Series Title: FASEB Journal vol:14 issue:10 pages:1389-99
Abstract: A synthetic gene encoding for HIV-1 integrase was designed to circumvent the intrinsic instability and the repressor elements present in the wild-type gene. High-level expression of HIV-1 integrase was obtained in various human cell lines independently of viral accessory proteins. A human 293T cell line was selected that stably expresses HIV-1 integrase and has growth kinetics comparable to the parental cell line. The enzyme was localized in the nucleus and remained stably associated with the chromosomes during mitosis. Lentiviral vector particles carrying the inactivating D64V mutation in the integrase gene were capable of stably transducing 293T cells when complemented in the producer cells with integrase expressed from the synthetic gene. When the cell line that stably expresses integrase was infected with the defective viral particles, complementation of integrase activity was detected as well. Expression of active HIV-1 integrase in human cells will facilitate the study of the interplay between host and viral factors during integration.
URI: 
ISSN: 0892-6638
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
Molecular Virology and Gene Therapy
Laboratory of Molecular Immunology (Rega Institute)
× corresponding author
# (joint) last author

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