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Title: Stimulation of the catalytic cycle of the Ca2+ pump of porcine plasma-membranes by negatively charged phospholipids
Authors: Lehotsky, Jan ×
Raeymaekers, Luc
Missiaen, Ludwig
Wuytack, Frank
De Smedt, Humbert
Casteels, Rik #
Issue Date: May-1992
Series Title: Biochimica et Biophysica Acta vol:1105 issue:1 pages:118-24
Abstract: The (Ca(2+)+Mg2+)-ATPase of the plasma membrane is activated by negatively charged phospholipids. The mechanism of this activation was investigated by studying the effect of negatively charged phospholipids on the steady-state phosphointermediate level and on the p-nitrophenylphosphatase activity. Both parameters were differentially affected by different acidic phospholipids. The level of phosphoprotein intermediate was not affected by phosphatidylserine (20% of total phospholipid), but it was increased by 60% by phosphatidylinositol 4-phosphate. Phosphatidylserine increased the p-nitrophenylphosphatase activity, whereas phosphatidylinositol 4-phosphate had no significant effect. It is suggested that phosphatidylinositol 4-phosphate mainly affects a reaction step which leads to accelerated formation of the phosphointermediate, whereas the action of phosphatidylserine would affect two reaction steps, one upstream and one downstream of the phosphointermediate.
ISSN: 0006-3002
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Pharmacology Section (-)
Laboratory of Molecular and Cellular Signaling
Laboratory of Cellular Transport Systems
× corresponding author
# (joint) last author

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