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Title: Modulation of TRPV4 gating by intra- and extracellular Ca2+
Authors: Watanabe, Hiroyuki ×
Vriens, Joris
Janssens, Annelies
Wondergem, Robert
Droogmans, Guy
Nilius, Bernd #
Issue Date: May-2003
Series Title: Cell calcium. vol:33 issue:5-6 pages:489-95
Abstract: We have studied the modulation of gating properties of the Ca2+-permeable, cation channel TRPV4 transiently expressed in HEK293 cells. The phorbol ester 4alphaPDD transiently activated a current through TRPV4 in the presence of extracellular Ca2+. Increasing the concentration of extracellular Ca2+ ([Ca2+](e)) reduced the current amplitude and accelerated its decay. This decay was dramatically delayed in the absence of [Ca2+](e). It was also much slower in the presence of [Ca2+](e) in a mutant channel, obtained by a point mutation in the 6th transmembrane domain, F707A. Mutant channels, containing a single mutation in the C-terminus of TRPV4 (E797), were constitutively open. In conclusion, gating of the 4alphaPDD-activated TRPV4 channel depends on both extra- and intracellular Ca2+, and is modulated by mutations of single amino acid residues in the 6th transmembrane domain and the C-terminus of the TRPV4 protein.
ISSN: 0143-4160
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Department of Cellular and Molecular Medicine - miscellaneous
Laboratory of Ion Channel Research (VIB-KU Leuven Center for Brain & Disease Research)
× corresponding author
# (joint) last author

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