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Title: Ca(2+) signals in Pmr1-GFP-expressing COS-1 cells with functional endoplasmic reticulum
Authors: Missiaen, Ludwig ×
Vanoevelen, Jo
Van Acker, Kristel
Raeymaekers, Luc
Parys, Jan
Callewaert, Geert
Wuytack, Frank
De Smedt, Humbert #
Issue Date: Jun-2002
Series Title: Biochemical and Biophysical Research Communications vol:294 issue:2 pages:249-53
Abstract: We studied the role of the Pmr1-containing Ca(2+) store in COS-1 cells endowed with a functional endoplasmic reticulum. Transfected cells could be recognized by using a green-fluorescent-protein (GFP)-tagged form of Pmr1. Pmr1-GFP fluorescence showed a typical juxtanuclear Golgi-like distribution. Pmr1-GFP-containing cells with functional endoplasmic reticulum responded to 100 microM ATP with baseline Ca(2+) spiking, while non-transfected cells produced an initial Ca(2+) peak followed by a long-lasting plateau. The Ca(2+) signal often appeared after a long latency in Pmr1-GFP-expressing cells. ATP-stimulated Pmr1-GFP-expressing cells with functional endoplasmic reticulum responded after a latency period to extracellular Ca(2+) with a regenerative Ca(2+) signal, while non-transfected control cells responded with an immediate slow rise in free cytosolic Ca(2+) concentration. These results demonstrate the importance of the Pmr1-containing Ca(2+) store in generating or modifying cellular Ca(2+) signals.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Laboratory of Cellular Transport Systems
Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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