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Title: Identification of inhibitor-2 as the ATP-mg-dependent protein phosphatase modulator
Authors: Yang, Shiaw-Der ×
Vandenheede, Jackie
Merlevede, Wilfried #
Issue Date: Dec-1981
Series Title: Journal of Biological Chemistry vol:256 issue:20 pages:10231-4
Abstract: In previous reports (Yang, S. D., Vandenheede, J. R., Goris, J., and Merlevede, W. (1980) J. Biol. Chem. 255, 11759-11767; Vandenheede, J. R., Yang, S.-D., and Merlevede, W. (1981) J. Biol. Chem. 256, 5894-5900), we have described two slightly different purification procedures for the isolation of the inactive ATP-Mg-dependent protein phosphatase (FC) which could be activated by a protein activator (FA) in the presence of ATP-Mg. Although the two procedures consistently produced FC preparations that showed very similar polyacrylamide gel patterns, the specific activity of the purified enzymes varied considerably. The preparations characterized by a rather low specific activity could be stimulated up to 10-fold when a titrated amount of inhibitor-2 was included during the FA and ATP-Mg-mediated activation. Inhibitor-2, only recently implicated as an inactivating protein for activated FC (Vandenheede, J. R., Goris, J., Yang, S.-D., Camps, T., and Merlevede, W. (1981) FEBS Lett. 127, 1-3) has now been identified as a modulator protein, necessary for the reversible activation of the protein phosphatase.
URI: 
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
× corresponding author
# (joint) last author

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