Title: Transepithelial fluctuation analysis of chloride secretion
Authors: Singh, Ashvani K ×
Schultz, Bruce D
Van Driessche, Willy
Bridges, Robert J #
Issue Date: Oct-2004
Series Title: Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society. vol:3 Suppl 2 pages:127-32
Abstract: Transepithelial fluctuation analysis (noise analysis) provides valuable information about the density and single-channel properties of ion channels in intact epithelia. Here we investigate cystic fibrosis transmembrane conductance regulator (CFTR)-dependent chloride (Cl-) secretion in T84 human colonic epithelia by inducing noise using the diarylsulfonylurea DASU-01, a low-affinity open-channel blocker of CFTR. Our data indicate that the apical membrane of maximally stimulated T84 epithelia has a very high Cl- conductance generated by approximately 7000 active CFTR channels per cell with open probability (Po) of approximately 0.4 and single-channel amplitude (i) of approximately 0.1 pA. Similar experiments might provide important information about how drugs regulate CFTR in intact epithelia.
ISSN: 1569-1993
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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