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Title: Periplasmic lysozyme inhibitor contributes to lysozyme resistance in Escherichia coli
Authors: Deckers, Daphne ×
Masschalck, Barbara
Aertsen, Abram
Callewaert, Lien
Van Tiggelen, C G M
Atanassova, Miroslava
Michiels, Christiaan #
Issue Date: May-2004
Publisher: Birkhäuser Verlag
Series Title: Cellular and Molecular Life Sciences vol:61 issue:10 pages:1229-1237
Abstract: The product of the Escherichia coli ORFan gene ykfE was recently shown to be a strong inhibitor of C-type lysozyme in vitro. The gene was correspondingly renamed ivy (inhibitor of vertebrate lysozyme), but its biological function in E. coli remains unknown. In this work, we investigated the role of Ivy in the resistance of E. coli to the bactericidal effect of lysozyme in the presence of outer-membrane-permeabilizing treatments. Both in the presence of lactoferrin (3.0 mg/ml) and under high hydrostatic pressure (250 MPa), the lysozyme resistance of E. coli MG1655 was decreased by knock-out of Ivy, and increased by overexpression of Ivy. However, knock-out of Ivy did not increase the lysozyme sensitivity of an E. coli MG1655 mutant previously described to be resistant to lysozyme under high pressure. These results indicate that Ivy is one of several factors that affect lysozyme resistance in E. coli, and suggest a possible function for Ivy as a host interaction factor in commensal and pathogenic E. coli.
ISSN: 1420-682X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Biochemistry Section (Medicine) (-)
× corresponding author
# (joint) last author

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