Title: The hydrolysis of lysophospholipids and nucleotides by autotaxin (NPP2) involves a single catalytic site
Authors: Gijsbers, Rik ×
Aoki, Junken
Arai, Hiroyuki
Bollen, Mathieu #
Issue Date: Mar-2003
Series Title: FEBS Letters vol:538 issue:1-3 pages:60-64
Abstract: Autotaxin (NPP2) is a tumor cell motility-stimulating factor that displays both a nucleotide pyrophosphatase/phosphodiesterase activity and a recently described lysophospholipase D activity. The hydrolysis of nucleotides is a metal-assisted reaction that occurs via a nucleotidylated threonine in the catalytic site. We show here that the catalytic site threonine and the metal-coordinating residues are also essential for the hydrolysis of lysophospholipids. In comparing the substrate specificity of NPP2 and the closely related NPP1 and NPP3, we found that only NPP2 displayed a lysophospholipase D activity, whereas NPP1 and NPP3 had a much higher nucleotide pyrophosphatase activity.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Virology and Gene Therapy
Biochemistry Section (Medicine) (-)
Laboratory of Biosignaling & Therapeutics
× corresponding author
# (joint) last author

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