This item still needs to be validated !
Title: Single-channel analysis of a large conductance channel in peroxisomes from rat liver
Authors: Lemmens, M ×
Verheyden, Kristine
Van Veldhoven, Paul P
Vereecke, Johan
Mannaerts, Guy
Carmeliet, Edward #
Issue Date: Oct-1989
Series Title: Biochimica et Biophysica Acta vol:984 issue:3 pages:351-9
Abstract: Native membranes and Triton X-100 solubilized integral membrane proteins of peroxisomes from rat liver were reconstituted in liposomes. With the patch clamp technique, a channel was detected with a conductance of 420 +/- 30 pS and a PK/PC1 of about 3. The channel in native membrane fractions was weakly voltage dependent, residing most of the time in an open state with the possibility to shift to different substates. Solubilization changed the kinetic properties. The channel became strongly voltage dependent and closed at voltages negative to -20 mV. The estimated diameter of the channel is about 1.7 nm and might explain, at least partially, the permeability properties of the peroxisomal membrane.
ISSN: 0006-3002
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmacology Section (-)
Physiology Section (-)
Laboratory of Lipid Biochemistry and Protein Interactions
Laboratory of Molecular and Cellular Signaling
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science