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Title: The nucleotide sequence of cDNA complementary to the C1 component of rat prostatic binding protein
Authors: Delaey, Bernard ×
Dirckx, Luc
Peeters, Benjamin
Volckaert, Guido
Mous, Jan
Heyns, Walter
Rombauts, Wilfried #
Issue Date: Aug-1983
Series Title: European Journal of Biochemistry vol:133 issue:3 pages:645-9
Abstract: The mRNA for component C1 of rat prostatic binding protein has been cloned and characterized. A partially purified mRNA fraction for this complex protein was reverse-transcribed into double-stranded cDNA and cloned into the PstI site of plasmid pBR 322. The 426-base-pair insert of the recombinant plasmid pC1A75 was completely sequenced. The coding region corresponds precisely to the 88 amino acid residues of C1 and in addition contains the information of a signal peptide of 23 residues. The 5' non-coding region counts only 19 nucleotides and is incomplete but the 3'-terminal non-coding part of 60 nucleotides extends into the poly(A) tail. Sequence analysis of other C1-positive clones indicates the presence of sequence rearrangements which must have occurred during the cloning procedure. Possible mechanisms for the generation of these cloning artefacts are discussed.
ISSN: 0014-2956
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Clinical and Experimental Endocrinology
Biochemistry Section (Medicine) (-)
× corresponding author
# (joint) last author

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