Title: Normal Ca2+ signalling in glutathione-depleted and dithiothreitol-treated HeLa cells
Authors: Missiaen, Ludwig ×
Parys, Jan
Bootman, M D
De Smedt, Humbert
Droogmans, Guillaume
Casteels, Rik #
Issue Date: Sep-1993
Series Title: Pflügers Archiv : European journal of physiology. vol:423 issue:5-6 pages:480-4
Abstract: We have investigated whether reducing agents and substances that interfere with glutathione metabolism would affect the histamine-induced rises in internal Ca2+ concentration ([Ca2+]i) in indo-1-loaded HeLa cells. Individual cells responded to 1 microM histamine with either baseline or sinusoidal Ca2+ oscillations, a single Ca2+ peak or a maintained elevation of the [Ca2+]i. Only a few cells did not respond. The sulphydryl reducing agent dithiothreitol (5 mM) did not affect these responses to histamine. A 24-h preincubation with 1 mM DL-buthionine (SR)-sulphoximine, which reduces the cellular glutathione content to less than 20% of its control value, affected neither these histamine responses, nor the [Ca2+]i rises after application of 2 microM thapsigargin. We conclude that oxidation of critical sulphydryl groups is not required for the normal response to histamine and also that glutathione plays no role in agonist-induced Ca2+ signalling in HeLa cells.
ISSN: 0031-6768
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Pharmacology Section (-)
Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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