Title: Analysis of a symbiosis-specific cytochrome P450 homolog in Rhizobium sp. BR816
Authors: Luyten, Ellen ×
Swinnen, Elfriede
Vlassak, Katrien
Verreth, Christel
Dombrecht, Bruno
Vanderleyden, Jozef #
Issue Date: Jul-2001
Publisher: APS Press
Series Title: Molecular Plant - Microbe Interactions vol:14 issue:7 pages:918-924
Abstract: Sequence analysis of the DNA region upstream of nodO in Rhizobium sp. BR816 revealed an open reading frame in which the deduced amino acid sequence shows homology with cytochrome P450. Because the BR816 P450 homolog shows 73% amino acid similarity with CYP127A1(Y4vG), which is identified on the symbiotic plasmid of Rhizobium sp. NGR234, it is named CYP127A2. Transcriptional analysis of CYP127A2 revealed high expression in bacteroids, whereas no or hardly any expression was observed under free-living conditions. Low-level, free-living expression, however was noticed when cells were grown microoxically at acid pH levels. A number of possible substrates that may induce P450 gene expression were analyzed, but only the addition of short-chain alcohols to cultures slightly increased CYP127A2 expression. High levels of CYP127A2 expression observed in bacteroids of a nifH mutant strain, which formed non-fixing nodules on bean, indicated that the genuine substrate for CYP127A2 is not a metabolite resulting from N2-fixation. Nevertheless, expression analysis pointed to a NifA- and sigma54-dependent transcription.
ISSN: 0894-0282
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Laboratory for Molecular Diagnosis
Microbial and Molecular Systems - miscellaneous
Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Technologiecluster Bioengineering Technologie
× corresponding author
# (joint) last author

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