Title: CAPS1 regulates catecholamine loading of large dense-core vesicles
Authors: Speidel, Dina ×
Bruederle, Cathrin E
Enk, Carsten
Voets, Thomas
Varoqueaux, Frederique
Reim, Kerstin
Becherer, Ute
Fornai, Francesco
Ruggieri, Stefano
Holighaus, Yvonne
Weihe, Eberhard
Bruns, Dieter
Brose, Nils
Rettig, Jens #
Issue Date: Apr-2005
Publisher: Cell Press
Series Title: Neuron vol:46 issue:1 pages:75-88
Abstract: CAPS1 is thought to play an essential role in mediating exocytosis from large dense-core vesicles (LDCVs). We generated CAPS1-deficient (KO) mice and studied exocytosis in a model system for Ca2+-dependent LDCV secretion, the adrenal chromaffin cell. Adult heterozygous CAPS1 KO cells display a gene dosage-dependent decrease of CAPS1 expression and a concomitant reduction in the number of docked vesicles and secretion. Embryonic homozygous CAPS1 KO cells show a strong reduction in the frequency of amperometrically detectable release events of transmitter-filled vesicles, while the total number of fusing vesicles, as judged by capacitance recordings or total internal reflection microscopy, remains unchanged. We conclude that CAPS1 is required for an essential step in the uptake or storage of catecholamines in LDCVs.
ISSN: 0896-6273
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Laboratory of Ion Channel Research (VIB-KU Leuven Center for Brain & Disease Research)
× corresponding author
# (joint) last author

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