Title: High-resolution measurement and calibration of Ca(2+)-transients using Indo-1 in guinea-pig atrial myocytes under voltage clamp
Authors: Callewaert, Geert ×
Lipp, P
Pott, L
Carmeliet, Edward #
Issue Date: Aug-1991
Series Title: Cell calcium. vol:12 issue:4 pages:269-77
Abstract: Spherical atrial myocytes obtained by enzymatic dispersion of hearts from adult guinea-pigs were loaded with the fluorescent Ca(2+)-indicator Indo-1 via patch-clamp pipettes. The dialysing solution additionally contained citrate (60 mM) as low-affinity ('linear') Ca(2+)-chelating compound in order to slow intracellular Ca(2+)-transients. Changes in Indo-1 fluorescence under voltage-clamp due to Ca(2+)-entry and/or release from the SR were calibrated using an in vivo procedure to determine the limiting fluorescence ratios. Sample recordings will be presented to demonstrate that components of a [Ca2+]i-transient due to entry via L-type Ca(2+)-channels and due to Ca(2+)-release from the SR can be directly visualized.
ISSN: 0143-4160
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
× corresponding author
# (joint) last author

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