Title: Isoform switching of the sarco(endo)plasmic reticulum Ca2+ pump during differentiation of BC3H1 myoblasts
Authors: De Smedt, Humbert ×
Eggermont, Jan
Wuytack, Frank
Parys, Jan
Van Den Bosch, Ludo
Missiaen, Ludwig
Verbist, Jan
Casteels, Rik #
Issue Date: May-1991
Series Title: Journal of Biological Chemistry vol:266 issue:11 pages:7092-5
Abstract: We have studied the expression of the gene 2 for the sarco(endo)plasmic reticulum Ca2+ pump (SERCA2) in BC3H1 cells. Myogenic differentiation not only activated the SERCA2 expression but it also induced an isoform switch. Undifferentiated myoblasts only expressed the SERCA2b isoform (non-muscle) whereas differentiated myocytes predominantly contained the SERCA2a isoform (cardiac/slow skeletal muscle). The isoform switch was documented by immunoblot analysis with isoform-specific antibodies. This observation was confirmed at the mRNA level by using antisense RNA probes specific for class 1 (SERCA2a) or class 2 (SERCA2b) messengers. The expression of the SERCA2a isoform after differentiation was accompanied by a decreased sensitivity of the Ca2+ uptake in permeabilized cells to the Ca2+ pump inhibitor thapsigargin.
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Research Group Experimental Neurology
Pharmacology Section (-)
Laboratory of Cell Transport (-)
Laboratory of Molecular and Cellular Signaling
Laboratory for Neurobiology (Vesalius Research Center)
Laboratory of Cellular Transport Systems
× corresponding author
# (joint) last author

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