Title: N-acetyl-sphingenine-1-phosphate is a potent calcium mobilizing agent
Authors: Gijsbers, Sofie ×
Mannaerts, Guy
Himpens, Bernard
Van Veldhoven, Paul P #
Issue Date: Aug-1999
Series Title: FEBS Letters vol:453 issue:3 pages:269-72
Abstract: Calcium mobilization induced by phosphorylated sphingoid bases was analyzed in calf pulmonary artery endothelial cells by confocal microscopy. A sphingenine-1-phosphate (SeP) analogue, N-acetyl-sphingenine-1-phosphate (N-C2-SeP), exogenously added to these cells, caused a fast and transient intracellular rise in calcium and was as potent as SeP. A minimal concentration of 0.6 nM for N-C2-SeP versus 1 nM for SeP was determined. The N-C2-SeP-induced Ca2+-signaling, like the response to SeP, was due to a release from thapsigargin-sensitive, ryanodine-insensitive, intracellular Ca2+-stores and not to a Ca2+-influx. N-C2-SeP can be considered as a truncated ceramide-phosphate, a lipid already reported to be mitogenic (Gomez-Munoz, A., Duffy, P.A., Martin, A., O'Brien, L., Byun, H.S., Bittman, R. and Brindley, D.N. (1995) Mol. Pharmacol. 47, 833-839), an effect that might be secondary to Ca2+-mobilization.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmacology Section (-)
Laboratory of Lipid Biochemistry and Protein Interactions
Laboratory of Molecular and Cellular Signaling
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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