Title: Polyamines and neomycin inhibit the purified plasma-membrane Ca2+ pump by interacting with associated polyphosphoinositides
Authors: Missiaen, Ludwig ×
Wuytack, Frank
Raeymaekers, Luc
De Smedt, Humbert
Casteels, Rik #
Issue Date: Nov-1989
Series Title: The Biochemical journal. vol:261 issue:3 pages:1055-8
Abstract: We investigated the effect of spermine, spermidine, putrescine and neomycin on the activity of the plasma-membrane Ca2+ pump and on its stimulation by negatively charged phospholipids and calmodulin. Millimolar concentrations of spermine and to a lesser extent of spermidine decreased the ATPase activity in the presence of phosphatidylinositol 4,5-bisphosphate (PIP2), without affecting the stimulation by phosphatidylinositol 4-phosphate (PIP). Sub-millimolar concentrations of neomycin inhibited the stimulation of the ATPase by PIP and by PIP2. Neomycin was more effective at the higher concentrations of PIP and PIP2. We discuss that these findings are compatible with the hypothesis that PIP and PIP2 bind to the ATPase and that several of these molecules have to be available to stimulate the ATPase.
ISSN: 0264-6021
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Pharmacology Section (-)
Laboratory of Cellular Transport Systems
Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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