Title: Pressure effects on lipid-protein interactions in (NA+ + K+)-ATPase
Authors: De Smedt, Humbert ×
Borghgraef, Roger
Ceuterick, F
Heremans, Karel #
Issue Date: Jan-1979
Publisher: Elsevier science bv
Series Title: Biochimica et Biophysica Acta vol:556 issue:3 pages:479-89
Abstract: The (Na+ + K+)-stimulated ATPase activity decreases with increasing pressure and a plot of the logarithm of the activity versus pressure shows a change in slope at a defined breakpoint pressure (Pb). The value of Pb increases linearly with increasing temperature. A dT/dP value of 27.7 +- 0.4 (S.D.) K/1000 atm is obtained. This is in very good agreement with the pressure shift for the melting transitions in phospholipids and aliphatic chains. This strongly indicates that an aliphatic chain melting process is involved in the breakpoint in the Arrhenius plot and pressure dependence of (Na+ + K+)-ATPase. The p-nitrophenyl phosphatase activity of this enzyme also decreases with pressure. In this case the plot of the logarithm of the activity versus pressure is linear without a break-point. The temperature dependence for (Na+ + K+)-ATPase was also studied in the presence of fluidizing drugs: desipramine and benzylalcohol. The presence of these drugs had no effect on the inflection point in the Arrhenius plot.
ISSN: 0006-3002
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Biochemistry, Molecular and Structural Biology Section
Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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