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Title: Cu2+ reveals different binding sites of amiloride and CDPC on the apical Na channel of frog skin
Authors: Flonta, Maria Luisa ×
Simaels, Jeannine
Mesotten, Dieter
Van Driessche, Willy #
Issue Date: Apr-1998
Series Title: Biochimica et Biophysica Acta vol:1370 issue:1 pages:169-74
Abstract: The effect of Cu2+ ions, present in the mucosal bathing solution, on the transepithelial short-circuit current (Isc) and conductance (Gt) and on the blocker-induced noise of apical Na channels, was studied on the isolated ventral skin of the frog Rana temporaria. Cu2+ effects were concentration-dependent, the full effect being reached at 50 micromol/l. Cu2+ increased Isc and Gt; this effect was eliminated by high concentrations of amiloride (30 micromol/l) and of CDPC (150 micromol/l). Cu2+ markedly reduced the corner frequency (fc) of the Na channel noise, while having virtually no effect on the fc of CDPC-induced noise. Cu2+ reduces the association rate constant of amiloride to the Na channel to one third; this effect is interpreted as indicating competition between Cu2+ and amiloride for the same (negatively charged) binding site on the channel, while CDPC appears to bind on a different site.
ISSN: 0006-3002
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Cellular and Molecular Medicine - miscellaneous
Laboratory of Intensive Care Medicine
× corresponding author
# (joint) last author

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