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Title: A synthetic peptide substrate specific for casein kinase I
Authors: Agostinis, Patrizia ×
Pinna, L A
Meggio, F
Marin, O
Goris, Jozef
Vandenheede, Jackie
Merlevede, Wilfried #
Issue Date: Dec-1989
Series Title: FEBS Letters vol:259 issue:1 pages:75-8
Abstract: The synthetic peptide, Asp-Asp-Asp-Glu-Glu-Ser-Ile-Thr-Arg-Arg, derived from the phosphorylation site of casein kinase-1 (CK-1) in beta-casein A(2), is readily phosphorylated by CK-1, but not by casein kinase-2(CK-2), cyclic AMP-dependent protein kinase, protein kinase C, phosphorylase kinase and protein kinase FA. Phosphorylation by CK-1 occurs only at Ser-6, Thr-8 being unaffected. The Km for the peptide is higher (1 mM) than for beta-casein A(2) (40 microM), while the Vmax is quite comparable. This is the first synthetic peptide substrate for CK-1 described so far, and can be used for the rapid and specific estimation of CK-1 activity in crude extracts.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Laboratory of Protein Phosphorylation and Proteomics
Laboratory of Cell Death Research & Therapy
× corresponding author
# (joint) last author

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