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Title: In vitro immunoneutralization of a cleaved prolactin variant: evidence for a local paracrine action of cleaved prolactin in the development of gonadotrophs and thyrotrophs in rat pituitary
Authors: Andries, Maria ×
Jacobs, G F
Tilemans, Diane
Denef, Carl #
Issue Date: Nov-1996
Publisher: Oxford University Press
Series Title: Journal of Neuroendocrinology vol:8 issue:2 pages:123-127
Abstract: We have previously isolated a cleaved prolactin variant, secreted by rat pituitary cells in culture, that stimulated [3H]thymidine incorporation into DNA in gonadotrophs and thyrotrophs when added to pituitary aggregate cell cultures of 14-day-old female rats. Using synthetic peptides homologous to the new C- and N-termini of the cleavage site, we made antisera recognizing this cleaved variant without significant cross reaction with native prolactin. Addition of these antisera to pituitary aggregate cell cultures decreased [3H]thymidine incorporation into DNA in gonadotrophs and thyrotrophs but not in the other pituitary cell types. These data are further evidence that this prolactin variant, cleaved between Tyr-145 and Leu-146, may have an important role as growth regulator of the gonadotrophs and thyrotrophs in the rat pituitary.
ISSN: 0953-8194
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmacology Section (-)
Laboratory of Biosignaling & Therapeutics
× corresponding author
# (joint) last author

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