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Title: Interleukin-8 activates microtubule-associated protein 2 kinase (ERK1) in human neutrophils
Authors: Van Lint, Johan ×
Van Damme, Jozef
Billiau, Alfons
Merlevede, Wilfried
Vandenheede, Jackie #
Issue Date: Nov-1993
Publisher: Kluwer academic publ
Series Title: Molecular and cellular biochemistry. vol:127-128 pages:171-7
Abstract: The signal transduction initiated by the human cytokine interleukin-8 (IL-8), the main chemotactic cytokine for neutrophils, was investigated and found to encompass the stimulation of protein kinases. More specifically, IL-8 caused a transient, dose and time dependent activation of a Ser/Thr kinase activity towards myelin basic protein (MBP) and the MBP-derived peptide APRTPGGRR patterned after the specific concensus sequence in MBP for ERK enzymes. The activated MBP kinase was furthermore identified as an extracellular signal regulated kinase (ERK1) based on several criteria such as substrate specificity, molecular weight, activation-dependent mobility shift, and recognition by anti-ERK antibodies. For comparison, the chemotactic response of neutrophils to a stimulus of bacterial origin (fMet-Leu-Phe or fMLP) was also examined and found to involve the activation of a similar ERK enzyme. The present data clearly indicate that in terminally differentiated, non-proliferating human cells, the MBP kinase/ERK activity can serve other purposes than mitogenic signaling, and that processes such as chemotaxis, induced by bacterial peptides as well as by human cytokines like IL-8, involve the regulation of ERK enzymes.
ISSN: 0300-8177
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Medicine - miscellaneous
Biochemistry Section (Medicine) (-)
Academic Center for General Practice
Laboratory of Molecular Immunology (Rega Institute)
Laboratory of Nephrology
Laboratory of Protein Phosphorylation and Proteomics
× corresponding author
# (joint) last author

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