Title: A study of dynamic properties in isolated myocardial cells by the laser diffraction method
Authors: Wussling, M ×
Schenk, W
Nilius, Bernd #
Issue Date: Mar-1988
Series Title: Journal of Molecular and Cellular Cardiology. vol:19 issue:9 pages:897-907
Abstract: Laser diffraction patterns were investigated from enzymatically isolated, unattached myocardial cells of guinea-pigs and mice. Experiments were performed at 2.5 mM Ca2+ and room temperature. The mean sarcomere length of resting guinea-pig and mouse myocardial cells amounted to about 1.83 micron and 1.75 micron, respectively. When paced with alternating intervals by field stimulation carefully selected ventricular cells showed transient phenomena. (1) The staircase following a rested state contraction was positive in the case of guinea-pig and negative in the case of mouse myocardial cells; (2) The rested state as compared to the steady state sarcomere shortening of guinea-pig and mouse cardiac myocytes amounted to 35% and 600%, respectively; (3) The interval strength curve of guinea-pig myocardial cells passed through a maximum which was 0.26 +/- 0.06 micron (mean +/- S.D.) at a pacing interval of 2 s whereas myocardial cells of mice showed a rise of shortening with increasing intervals reaching a maximum at the rested state (0.24 +/- 0.08 micron). Results were similar to those obtained from multicellular preparations. We conclude therefore, dynamic properties of multicellular preparations are nicely reflected at the sarcomere level.
ISSN: 0022-2828
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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