Title: Existence of a calcium-dependent potassium channel in the membrane of cow cardiac Purkinje cells
Authors: Callewaert, Geert ×
Vereecke, Johan
Carmeliet, Edward #
Issue Date: Jul-1986
Series Title: Pflügers Archiv : European journal of physiology. vol:406 issue:4 pages:424-6
Abstract: Single-channel currents were recorded in the membrane of cow cardiac Purkinje cells using the patch-clamp technique. Recordings from cell-attached and cell-free patches demonstrated large outward single-channel currents associated with depolarizing voltage-clamp pulses. The time course of the reconstructed mean current showed a rapid activation phase followed by a slower inactivation following a single exponential time course with a time-constant in the range 30 ms to 100 ms. The current-voltage relation of the channel was linear in the voltage range between +10 mV and +110 mV with a slope conductance of 120 pS in 10.8 mM external K. The results indicated that the channel is selective for K ions. In inside-out patches, when the internal Ca activity was raised from 0.01 microM to 1 microM, the frequency of opening of the K channel during a depolarizing pulse was markedly increased, indicating Ca-dependence of these channels. The relation between this ion channel and the previously described transient outward current in cow Purkinje fibres is discussed. In sheep Purkinje cells a channel, carrying a transient outward current, with different properties was found.
ISSN: 0031-6768
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Laboratory of Molecular and Cellular Signaling
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science