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Title: On the mechanism of activation of the ATP X Mg(II)-dependent phosphoprotein phosphatase by kinase FA
Authors: Jurgensen, S ×
Shacter, E
Huang, C Y
Chock, P B
Yang, Shiaw-Der
Vandenheede, Jackie
Merlevede, Wilfried #
Issue Date: Jun-1984
Series Title: Journal of Biological Chemistry vol:259 issue:9 pages:5864-70
Abstract: The mechanism of activation of the Mg(II) X ATP-dependent phosphatase by the kinase FA has been investigated. The inactive protein phosphatase can be represented as FC X M where FC is the inactive catalytic component and M is the heat-stable modulator protein (also known as inhibitor-2). Phosphorylation of the modulator protein is demonstrated during activation of FC X M. In addition, continuous ATP hydrolysis during the activation is observed. This suggests that a cyclic phosphorylation-dephosphorylation reaction is continuously occurring during the activation. It is proposed that phosphorylation of the modulator protein causes an isomerization in FC to generate an active phosphatase. The activated phosphatase is capable of dephosphorylating the phosphorylated modulator. Upon dephosphorylation of modulator, the active phosphatase returns to its inactive form via a slow isomerization.
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
× corresponding author
# (joint) last author

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