Title: Kinetics of the non-specific calcium leak from non-mitochondrial calcium stores in permeabilized A7r5 cells
Authors: Missiaen, Ludwig ×
De Smedt, Humbert
Parys, Jan
Raeymaekers, Luc
Droogmans, Guillaume
Van Den Bosch, Ludo
Casteels, Rik #
Issue Date: Sep-1996
Series Title: The Biochemical journal. vol:317 ( Pt 3) pages:849-53
Abstract: We have investigated the detailed kinetics of the passive Ca2+ leak from non-mitochondrial Ca2+ stores in permeabilized A7r5 cells. The decrease in the content of stored Ca2+ in the presence of 2 microM thapsigargin deviated from a single-exponential curve in the initial phase of the efflux. The deviation persisted after correcting this efflux for passively bound Ca2+. The non-single-exponential nature of the spontaneous release also occurred when the initial store Ca2+ content was reduced to 40% of its original value by pretreatment with 200 nM inositol 1,4,5-trisphosphate (InsP3). The passive Ca2+ leak could be modelled by two exponential curves with discrete rate constants of 0.06 min-1 and 0.98 min-1, and not by any other type of non-exponential decay. We concluded that individual store units are heterogeneous with respect to their passive Ca2+ permeability. This non-exponential nature of the passive Ca2+ release is unrelated to the non-single-exponential InsP3-induced Ca2+ release.
ISSN: 0264-6021
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Physiology Section (-)
Research Group Experimental Neurology
Pharmacology Section (-)
Laboratory of Molecular and Cellular Signaling
Laboratory for Neurobiology (Vesalius Research Center)
× corresponding author
# (joint) last author

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